abstract：:cDNA clones coding for rabbit prolactin were isolated from a pituitary library using a rat prolactin RNA probe. One cDNA contained 873 bases including the entire coding sequence of rabbit prolactin, its signal peptide and the 5' and 3' untranslated regions of 44 and 145 nucleotides respectively. The deduced amino acid...

journal_title：Journal of molecular endocrinology

authors： Gabou L,Boisnard M,Gourdou I,Jammes H,Dulor JP,Djiane J

更新日期：1996-02-01 00:00:00

abstract：:Parathyroid hormone-related peptide (PTHrP) is thought to be responsible for hypercalcemia in some patients with malignant tumors. The PTHrP gene has seven exons, giving rise to three types of PTHrP isoform through alternative splicing. We studied the expression of mRNAs in 14 human cell lines using the reverse transc...

journal_title：Journal of molecular endocrinology

authors： Nakamura M,Hashimoto T,Furuyama J,Kakudo K

更新日期：1995-12-01 00:00:00

abstract：:Relaxin is a peptide hormone which is produced in human reproductive tissues including the ovary and prostate gland. Little is known of the molecular events regulating relaxin gene transcription. We have studied this question using gene transfer of relaxin promoter/reporter gene constructs into a relaxin-expressing ce...

journal_title：Journal of molecular endocrinology

authors： Gunnersen JM,Roche PJ,Tregear GW,Crawford RJ

更新日期：1995-10-01 00:00:00

abstract：:It has been suggested that the thyroid itself may contribute to the inflammatory process observed in autoimmune thyroiditis by releasing the cytokines interleukin-1 alpha (IL-1 alpha), interleukin-6 (IL-6) and interleukin-8 (IL-8), but studies of cytokine gene expression in thyrocytes have been limited and conflicting...

journal_title：Journal of molecular endocrinology

authors： Watson PF,Pickerill AP,Davies R,Weetman AP

更新日期：1995-08-01 00:00:00

abstract：:We investigated the mechanisms by which corticosteroids regulate the expression of the mineralocorticoid receptor (MR) in neurones. Aldosterone and dexamethasone produced a dose-dependent increase of MR and mRNA levels in cultured primary hippocampal neurones. Transient transfection of neuroblastoma cells showed that ...

journal_title：Journal of molecular endocrinology

authors： Castrén M,Trapp T,Berninger B,Castrén E,Holsboer F

更新日期：1995-06-01 00:00:00

abstract：:Several authors have shown that angiotensin II stimulates hepatic angiotensinogen synthesis in vivo, ex vivo and in vitro. In previous studies we have demonstrated that this effect of angiotensin II depends mainly on a transient inhibition of adenylyl cyclase and is the consequence of a stabilization of angiotensinoge...

journal_title：Journal of molecular endocrinology

authors： Klett C,Bader M,Schwemmle M,Ganten D,Hackenthal E

更新日期：1995-04-01 00:00:00

abstract：:Porcine LH receptor ectodomain was overexpressed in insect cells and lepidopteran larvae using the recombinant baculovirus expression system. A low multiplicity of infection yielded the largest active production, of approximately 10(7) receptors/cell or 3 micrograms active receptor/mg total protein in infected cells. ...

journal_title：Journal of molecular endocrinology

authors： Pajot-Augy E,Couture L,Bozon V,Remy JJ,Biache G,Severini M,Huet JC,Pernollet JC,Salesse R

更新日期：1995-02-01 00:00:00

abstract：:Seven bovine follistatin (FS) cDNA clones were isolated from a bovine ovarian follicle cDNA library. The predicted amino acid sequences revealed that six of the cDNA clones represented an FS precursor of 344 amino acids which corresponded to a mature FS of 315 amino acids (FS 315), with one cDNA clone containing the e...

journal_title：Journal of molecular endocrinology

authors： Saleh M,Garcia S,Mercer JE,Findlay JK

更新日期：1994-12-01 00:00:00

abstract：:Human galanin (hGal) is an important neuro-modulator present in the brain, gastrointestinal system and the hypothalamo-pituitary axis. A specific receptor for hGal has been identified in various areas in human brain. A single class of high affinity binding sites was found on plasma membranes of the amygdala (Kd 0.23 n...

journal_title：Journal of molecular endocrinology

authors： Walli R,Schäfer H,Morys-Wortmann C,Paetzold G,Nustede R,Schmidt WE

更新日期：1994-12-01 00:00:00

abstract：:Alkaline (pI 8.6-7.5) and neutral (pI 7.0-6.0) isoforms of human TSH have been isolated from a highly purified intrapituitary preparation by isoelectric focusing and compared for their respective actions on thyroid cell proliferation. Both TSH isoforms displayed the same ability to bind to porcine thyroid membranes as...

journal_title：Journal of molecular endocrinology

authors： Medri G,Sergi I,Papandréou MJ,Beck-Peccoz P,Verrier B,Ronin C

更新日期：1994-10-01 00:00:00

abstract：:Insulin is important for optimal fetal and neonatal growth and development. Its continued availability is due, in part, to ongoing islet cell growth within the pancreas. IGFs and IGF-binding proteins (IGFBPs) have been implicated as paracrine regulators of islet cell growth within the developing pancreas. The purpose ...

journal_title：Journal of molecular endocrinology

authors： Hogg J,Hill DJ,Han VK

更新日期：1994-08-01 00:00:00

abstract：:Translocation of protein kinase C (PKC) from the cytosol to the plasma membranes is believed to reflect activation of the enzyme. We have studied translocation of PKC in lactotroph-enriched anterior pituitary cell cultures by measuring the incorporation of gamma-32P from [gamma-32P]ATP into a synthetic peptide substra...

journal_title：Journal of molecular endocrinology

authors： Mau SE,Saermark T,Vilhardt H

更新日期：1994-06-01 00:00:00

abstract：:A transgenic mouse line (EGF/Tag) has been established in which expression of SV40 T-antigen is directed by a 5.5 kb fragment of the 5'-flanking region of the mouse epidermal growth factor (EGF) gene. Of the two principal sites of EGF expression in mice, submaxillary gland and kidney, T-antigen mRNA and protein were d...

journal_title：Journal of molecular endocrinology

authors： Pascall IC,Surani MA,Barton SC,Vaughan TJ,Brown KD

更新日期：1994-06-01 00:00:00

abstract：:This study examined fetal steroidogenic enzyme expression and function during pregnancy in the pig. Northern and Western analyses were performed to detect the cytochrome P450 enzyme 17 alpha-hydroxylase/17-20 lyase (P450c17) and that for cholesterol side-chain cleavage (P450scc), as well as 3 beta-hydroxysteroid dehyd...

journal_title：Journal of molecular endocrinology

authors： Conley AJ,Rainey WE,Mason JI

更新日期：1994-04-01 00:00:00

abstract：:Two forms of prolactin (tiPRLI and tiPRLII), with only 69% sequence identity, have been previously described in the cichlid fish tilapia (Oreochromis species). In the present study we have attempted to investigate the biological activity of these two prolactin forms during adaptation to a hyperosmotic environment. For...

journal_title：Journal of molecular endocrinology

authors： Auperin B,Rentier-Delrue F,Martial JA,Prunet P

更新日期：1994-02-01 00:00:00

abstract：:We studied the effect of adenosine on prolactin secretion by the anterior pituitary, and the transduction mechanisms whereby the purine exerts its action. Adenosine inhibited prolactin release in basal and in vasoactive intestinal peptide (VIP)- or TRH-stimulated conditions. Pertussis toxin pretreatment reduced the in...

journal_title：Journal of molecular endocrinology

authors： Scorziello A,Landolfi E,Grimaldi M,Meucci O,Ventra C,Avallone A,Postiglione A,Schettini G

更新日期：1993-12-01 00:00:00

abstract：:The cloning and characterization of the mouse TRH receptor (TRH-R) gene revealed an untranslated exon (exon 1), a single intron and an upstream dinucleotide repeat sequence (d(TG)16.d(AG)21) in the 5' untranslated region (UTR). The coding region was contained almost entirely on a second exon (exon 2), with the final a...

journal_title：Journal of molecular endocrinology

authors： Duthie SM,Taylor PL,Eidne KA

更新日期：1993-10-01 00:00:00

abstract：:We have generated hybridomas which secrete monoclonal antibodies to the AT1 subtype of the angiotensin II receptor (AT1 receptor). These were obtained after immunization of Balb C/c mice with synthetic peptides representing sequences from either the extracellular domain (residues 8-17) or the intracellular domain (res...

journal_title：Journal of molecular endocrinology

authors： Barker S,Marchant W,Ho MM,Puddefoot JR,Hinson JP,Clark AJ,Vinson GP

更新日期：1993-10-01 00:00:00

abstract：:The peroxisome proliferator-activated receptor (PPAR) is a member of the steroid hormone receptor superfamily and is activated by a variety of fibrate hypolipidaemic drugs and non-genotoxic rodent hepatocarcinogens that are collectively termed peroxisome proliferators. A key marker of peroxisome proliferator action is...

journal_title：Journal of molecular endocrinology

authors： Issemann I,Prince RA,Tugwood JD,Green S

更新日期：1993-08-01 00:00:00

abstract：:Inhibition of aromatase, a key enzyme in the biosynthesis of oestradiol-17 beta, by the addition of 1,4,6-androstatrien-3,17-dione resulted in a significant increase in the levels of immunoreactive human chorionic gonadotrophin (hCG) in the medium and tissue. This increase was partially reversed by the simultaneous ad...

journal_title：Journal of molecular endocrinology

authors： Sharma SC,Purohit P,Rao AJ

更新日期：1993-08-01 00:00:00

abstract：:Localization of inhibin/activin subunit mRNAs within the macaque ovary from the immediate pre-ovulatory period of the menstrual cycle, when serum immunoreactive inhibin begins to rise, to day 9 of the luteal phase, when serum inhibin concentrations are maximal, was investigated using in-situ hybridization. Ovaries wer...

journal_title：Journal of molecular endocrinology

authors： Fraser HM,Lunn SF,Cowen GM,Saunders PT

更新日期：1993-06-01 00:00:00

abstract：:We have analysed the expression of the IGF-I receptor gene in lymphocytes of patients with low levels of circulating IGF-I (four patients with isolated GH deficiency (IGHD) and one Laron-type dwarf (LTD)) in comparison with a control group exhibiting normal serum IGF-I levels and endocrine profiles. 125I-Labelled IGF-...

journal_title：Journal of molecular endocrinology

authors： Eshet R,Werner H,Klinger B,Silbergeld A,Laron Z,LeRoith D,Roberts CT Jr

更新日期：1993-04-01 00:00:00

abstract：:This study determined whether twice-daily intrauterine injections of ovine conceptus secretory proteins (oCSP) containing type-I trophoblast interferon (25 micrograms/uterine horn) from day 11 to day 15 post-oestrus (oestrus = day 0) could alter the binding capacities of endometrial receptors for oxytocin, progesteron...

journal_title：Journal of molecular endocrinology

authors： Mirando MA,Harney JP,Zhou Y,Ogle TF,Ott TL,Moffatt RJ,Bazer FW

更新日期：1993-04-01 00:00:00

abstract：:The hyperglycaemia-inducing sulphonamide diazoxide has been previously shown to mediate its effects upon insulin secretion by opening K+ channels and hyperpolarizing the beta-cell membrane. The target site has been characterized as the ATP-regulated K+ (K+ATP) channel protein. In the present study, a detailed investig...

journal_title：Journal of molecular endocrinology

authors： Jaggar JH,Harding EA,Ayton BJ,Dunne MJ

更新日期：1993-02-01 00:00:00

abstract：:Obesity is likely to be a multifactorial disease with an important genetic component. Animal models of genetic and experimentally induced obesity suggest that glucocorticoid receptor (GR) activity plays a role in the aetiology and maintenance of the obese state. Glucocorticoid activity appears to be essential for the ...

journal_title：Journal of molecular endocrinology

authors： Weaver JU,Hitman GA,Kopelman PG

更新日期：1992-12-01 00:00:00

abstract：:DNA encoding the N-terminal 415 residues of the human thyrotrophin receptor (predicted to code for the large extracellular region) was introduced into Chinese hamster ovary (CHO) cells using the glutamine synthetase/cytomegalovirus amplifiable expression system, and into E. coli using the pGEX-3X expression vector. Su...

journal_title：Journal of molecular endocrinology

authors： Harfst E,Johnstone AP,Nussey SS

更新日期：1992-12-01 00:00:00

abstract：:The presence of retinoic acid receptor (RAR) alpha, beta and gamma mRNA was examined in 16 different kinds of rat tissue using the highly sensitive reverse transcriptase-polymerase chain reaction technique. The data demonstrated that each tissue expressed at least two types of RAR mRNA. Among the three types of RAR mR...

journal_title：Journal of molecular endocrinology

authors： Wan YJ,Wang L,Wu TC

更新日期：1992-12-01 00:00:00

abstract：:Several hormone agonists exert their physiological actions by triggering an inositol phospholipid-Ca2+ signalling cascade and cytosolic alkalinization. Although calcium ionophores have been used extensively to probe the role of Ca2+ in the regulation of steroidogenesis in granulosa cells, the precise relationship betw...

journal_title：Journal of molecular endocrinology

authors： Asem EK,Li M,Tsang BK

更新日期：1992-08-01 00:00:00

abstract：:Hypogonadal (hpg) mutant mice, with a congenital deficiency of hypothalamic gonadotrophin-releasing hormone (GnRH), and testicular feminized (tfm) mice, which lack a functional androgen receptor, were used to study the effects of the potent GnRH agonist 'Zoladex' (ICI 118630; D-Ser (Bu(t))6, Azgly10-GnRH) on pituitary...

journal_title：Journal of molecular endocrinology

authors： Scott IS,Bennett MK,Porter-Goff AE,Harrison CJ,Cox BS,Grocock CA,O'Shaughnessy PJ,Clayton RN,Craven R,Furr BJ

更新日期：1992-06-01 00:00:00

abstract：:Transformation of Escherichia coli cells with a recombinant plasmid containing modified mouse prolactin (mPRL) cDNA and a pKK223-3 vector resulted in efficient expression of mPRL protein. Cloned mPRL cDNA was modified by removing the 5' non-translating sequence as well as the sequence which encoded the signal peptide ...

journal_title：Journal of molecular endocrinology

authors： Yamamoto M,Harigaya T,Ichikawa T,Hoshino K,Nakashima K

更新日期：1992-04-01 00:00:00

abstract：:Granulosa cells were prepared from small follicles (less than 3 mm) from the ovaries of 5-month-old gilts. They were cultured in plastic dishes coated with a synthetic adhesion peptide in a chemically defined medium supplemented with 2% serum substitute. After 3 days of culture, the cells reached confluence and expres...

journal_title：Journal of molecular endocrinology

authors： Goxe B,Salesse R,Remy JJ,Genty N,Garnier J

更新日期：1992-04-01 00:00:00

abstract：:The development of an efficient expression system for insulin-like growth factor-I (IGF-I) in Escherichia coli as a fusion protein is described. The fusion protein consists of an N-terminal extension made up of the first 46 amino acids of methionyl porcine GH ([Met1]-pGH) followed by the dipeptide Val-Asn. The latter ...

journal_title：Journal of molecular endocrinology

authors： King R,Wells JR,Krieg P,Snoswell M,Brazier J,Bagley CJ,Wallace JC,Ballard FJ,Ross M,Francis GL

更新日期：1992-02-01 00:00:00

abstract：:Two marsupial casein genes have been isolated from a tammar wallaby (Macropus eugenii) mammary gland cDNA library. Comparisons of the tammar alpha- and beta-casein genes with their eutherian homologous reveal extensive divergence at the levels of nucleotide and amino acid sequences. Regions of similarity between the t...

journal_title：Journal of molecular endocrinology

authors： Collet C,Joseph R,Nicholas K

更新日期：1992-02-01 00:00:00

abstract：:Using the fluorescent indicators 2',7'-bis(2-carboxyethyl)-5'-(6')-carboxyfluorescein and Oxonol V to monitor intracellular pH (pHi) and cell membrane potential respectively, we have investigated the involvement of H(+)-dependent ATPase and H(+)-dependent K+ channels in the recovery of the rat thyroid cell strain FRTL...

journal_title：Journal of molecular endocrinology

authors： Woods DJ,Soden J,Bidey SP

更新日期：1992-02-01 00:00:00

abstract：:The constitutive expression of 25-hydroxyvitamin D3-24-hydroxylase (25-(OH)D3-24-hydroxylase) activity has been studied in an adherent variant (Ad-HL60) of the human promyelomonocytic leukaemia cell line HL60. The Ad-HL60 cells have a more differentiated phenotype than the non-adherent cells from which they were deriv...

journal_title：Journal of molecular endocrinology

authors： Hayes ME,Bayley D,Mawer EB

更新日期：1991-12-01 00:00:00

abstract：:Human thyroid hormone receptor (c-erb A protein) produced by Escherichia coli expression vector plasmid was purified sequentially using polyethylenimine precipitation of DNA, hydroxylapatite column chromatography, ammonium sulphate precipitation, Sephacryl S-300 gel filtration and mono Q-Sepharose column chromatograph...

journal_title：Journal of molecular endocrinology

authors： Ichikawa K,Hashizume K,Nishii Y,Takeda T,Kobayashi M,Suzuki S,Yamada T

更新日期：1991-10-01 00:00:00

abstract：:Angiotensin II (AII) receptors were identified in rat tissue membranes by specific binding of 125I-labelled AII. Using an isoelectric focusing technique, two forms of the high-affinity AII receptor were identified in rat adrenal zona glomerulosa and liver membranes. These migrated to isoelectric points (pI) 6.8 and 6....

journal_title：Journal of molecular endocrinology

authors： Jimenez E,Marsigliante S,Barker S,Hinson JP,Vinson GP

更新日期：1991-08-01 00:00:00

abstract：:Differences in binding and structural properties of ovine testicular FSH and LH receptors were investigated. The ovine FSH receptor did not discriminate between FSH of different species, although equine FSH was more reactive. In the same tissue, however, the LH receptor showed marked preference for ovine and bovine LH...

journal_title：Journal of molecular endocrinology

authors： Yarney TA,Sairam MR

更新日期：1991-06-01 00:00:00

abstract：:Protein kinase C (PKC) has been identified in islets of Langerhans and insulin-secreting tumour cells. Diacylglycerols (DAGs, the endogenous PKC activators) are generated in response to insulin secretagogues, although nutrient and non-nutrient secretagogues generate DAGs of different compositions and of different pote...

journal_title：Journal of molecular endocrinology

authors： Jones PM,Persaud SJ,Howell SL

更新日期：1991-04-01 00:00:00

abstract：:A prolactin cDNA was cloned from a cDNA expression library constructed from total RNA of bullfrog (Rana catesbeiana) adenohypophyses by immunoscreening with antiserum against bullfrog prolactin. The cDNA clone thus obtained contained a 249 bp insert. Using this clone as a probe, plaque hybridizations were performed an...

journal_title：Journal of molecular endocrinology

authors： Takahashi N,Yoshihama K,Kikuyama S,Yamamoto K,Wakabayashi K,Kato Y

更新日期：1990-12-01 00:00:00